Adoptive Transfer of Lung Antigen Presenting Cells

Xiaofeng  Zhou; Bethany  Moore

doi:10.21769/bioprotoc.2182

Bio-Protocol (Mar 2017)

Adoptive Transfer of Lung Antigen Presenting Cells

Xiaofeng Zhou,
Bethany Moore

Affiliations

Xiaofeng Zhou: Department of Internal Medicine, Pulmonary and Critical Care Medicine Division, University of Michigan, Ann Arbor, Michigan, USA
Bethany Moore: Department of Internal Medicine, Pulmonary and Critical Care Medicine Division, University of Michigan, Ann Arbor, Michigan, USA

DOI: https://doi.org/10.21769/bioprotoc.2182
Journal volume & issue: Vol. 7, no. 6

Abstract

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Our protocol describes adoptive transfer of antigen presenting cells (APCs) isolated from the lungs by enzymatic digestion and magnetic enrichment. This protocol can be used to study APC functions and trafficking.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

About the journal