Differentiation of Mscs into Osteoblasts on a Porous 3D -Carrier of Poly-3-Hydroxybutyrate

Abstract

The study describes preparation and testing of porous 3D implants of natural degradable polymer of 3-hydroxybutyric acid [P(3HB)] for regeneration of bone tissue defects. The ability of the 3D carriers to favor attachment and facilitate proliferation and directed differentiation into osteoblasts of mesenchymal stem cells (MSCs) was studied in the culture of MSCs isolated from bone marrow and adipose tissue. MSC differentiation into osteoblasts was confirmed by measurements of the activity of alkaline phosphatase, gene expression of the marker of bone protein BGP (glutamic protein participating in mineralization) and measurements of intracellular precipitates of calcium and phosphorus salts

Keywords

• cellular carrier
• poly-3-hydroxybutyrate
• mesenchymal stem cells
• differentiation
• osteoblasts