Matrix-assisted laser desorption ionization mass spectrometry profiling of plasma exosomes evaluates osteosarcoma metastasis
Zhenzhen Han,
Cheng Peng,
Jia Yi,
Yiwen Wang,
Qi Liu,
Yi Yang,
Shuping Long,
Liang Qiao,
Yuhui Shen
Affiliations
Zhenzhen Han
Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200000, China; Department of Chemistry, Fudan University, Shanghai 200433, China
Cheng Peng
Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200000, China
Jia Yi
Department of Chemistry, Fudan University, Shanghai 200433, China
Yiwen Wang
Department of Chemistry, Fudan University, Shanghai 200433, China
Qi Liu
Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200000, China
Yi Yang
Department of Chemistry, Fudan University, Shanghai 200433, China
Shuping Long
Department of Clinical Laboratory Medicine, Shanghai Tenth People's Hospital of Tongji University, Shanghai, China
Liang Qiao
Department of Chemistry, Fudan University, Shanghai 200433, China; Corresponding author
Yuhui Shen
Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200000, China; Corresponding author
Summary: Osteosarcoma is the most common primary sarcoma of bone among adolescents, often characterized by early lung metastasis resulting in high mortality. Recently, exosomes have been used in liquid biopsy to monitor tumors. Herein, we used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to profile human plasma exosomes for the evaluation of osteosarcoma lung metastasis. Forty patients with osteosarcoma with (n = 20) or without (n = 20) lung metastasis as well as 12 heathy controls were recruited. Exosomes were isolated from human plasma for MALDI-TOF MS analysis. Multivariate statistical analyses were performed based on the MALDI-TOF mass spectra. The strategy can efficiently differentiate osteosarcomas from healthy controls and further discriminate osteosarcoma lung metastasis from non-lung metastasis. We identified seven exosomal proteins as potential biomarkers of osteosarcoma lung metastasis. The proposed method holds great promise to clinically diagnose osteosarcoma and monitor osteosarcoma lung metastasis.
