DNA replication origins retain mobile licensing proteins

Humberto Sánchez; Kaley McCluskey; Theo van Laar; Edo van Veen; Filip M. Asscher; Belén Solano; John F. X. Diffley; Nynke H. Dekker

doi:10.1038/s41467-021-22216-x

Nature Communications (Mar 2021)

DNA replication origins retain mobile licensing proteins

Humberto Sánchez,
Kaley McCluskey,
Theo van Laar,
Edo van Veen,
Filip M. Asscher,
Belén Solano,
John F. X. Diffley,
Nynke H. Dekker

Affiliations

Humberto Sánchez: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology
Kaley McCluskey: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology
Theo van Laar: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology
Edo van Veen: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology
Filip M. Asscher: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology
Belén Solano: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology
John F. X. Diffley: Chromosome Replication Laboratory, Francis Crick Institute
Nynke H. Dekker: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology

DOI: https://doi.org/10.1038/s41467-021-22216-x
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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Eukaryotic DNA replication is regulated to ensure copying of the genome (only) once per cell cycle. Here the authors, using optical trapping and confocal microscopy, demonstrate the dynamics of the origin recognition complex and subsequent intermediates that lead up to the loading of an MCM helicase onto DNA.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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