Analysis of Methanotroph Populations from Various Sources for Production of High-Value Products

Lisa Stephanie H. Dizon; Robert S. Bertrand; William E. Holmes; Rafael A. Hernandez; Dhan Lord B. Fortela; Andre Chistoserdov; Mark E. Zappi; Emmanuel D. Revellame

doi:10.3390/ASEC2022-13953

Engineering Proceedings (Jan 2023)

Analysis of Methanotroph Populations from Various Sources for Production of High-Value Products

Lisa Stephanie H. Dizon,
Robert S. Bertrand,
William E. Holmes,
Rafael A. Hernandez,
Dhan Lord B. Fortela,
Andre Chistoserdov,
Mark E. Zappi,
Emmanuel D. Revellame

Affiliations

Lisa Stephanie H. Dizon: The Energy Institute of Louisiana, University of Louisiana at Lafayette, Lafayette, LA 70504, USA
Robert S. Bertrand: The Energy Institute of Louisiana, University of Louisiana at Lafayette, Lafayette, LA 70504, USA
William E. Holmes: The Energy Institute of Louisiana, University of Louisiana at Lafayette, Lafayette, LA 70504, USA
Rafael A. Hernandez: The Energy Institute of Louisiana, University of Louisiana at Lafayette, Lafayette, LA 70504, USA
Dhan Lord B. Fortela: The Energy Institute of Louisiana, University of Louisiana at Lafayette, Lafayette, LA 70504, USA
Andre Chistoserdov: The Energy Institute of Louisiana, University of Louisiana at Lafayette, Lafayette, LA 70504, USA
Mark E. Zappi: The Energy Institute of Louisiana, University of Louisiana at Lafayette, Lafayette, LA 70504, USA
Emmanuel D. Revellame: The Energy Institute of Louisiana, University of Louisiana at Lafayette, Lafayette, LA 70504, USA

DOI: https://doi.org/10.3390/ASEC2022-13953
Journal volume & issue: Vol. 31, no. 1
p. 30

Abstract

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Methanotrophs are bacteria that can consume methane as their sole carbon and energy source to produce a wide variety of high-value products such as lipids, biopolymers, ectoine, and single cell proteins (SCPs). Collected samples from various sources were subjected to DNA extraction followed by 16S rRNA analysis to determine the identity and relative abundance of their microbial population. Several taxa of methanotrophs were detected in the samples including Type I (Methylobacter), Type X (Methylocaldum), Type II (Methylocystis, Methylosinus, and Beijerinckia), and Type III (Verrucomicrobium). This paper expounds the effects of environmental/cultivation conditions on the growth and population of different types of methanotrophs. The results could be used to systematically identify source(s) of natural consortia that can be enriched and developed to produce specific target product(s) under a given cultivation conditions/limitations.

Published in Engineering Proceedings

ISSN: 2673-4591 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Engineering (General). Civil engineering (General): Engineering machinery, tools, and implements
Website: https://www.mdpi.com/journal/engproc

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