Immunolocalization of lipoxygenase in the microspore of Gagea lutea (L.) Ker.-Gaw.

Abstract

Localization of lipoxygenase (LOX) in the microspore of Gagea lutea (L.) Ker.-Gaw. was investigated with the immunogold labelling method. The enzyme was found in the cytoplasm, nucleus and sporoderm. The most intensive reaction was observed in the cytoplasm, where the immunogold particles were sometimes grouped into clusters of several or more and showed the highest density. The smallest amount of particles occured in the sporoderm. The role of lipoxygenase in the microspore is discussed.

Keywords

• classification system
• plant community
• similarity
• Sphagnum fimbriatum