Nature Communications (Jan 2022)

Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing

  • Matthew J. Tarnowski,
  • Thomas E. Gorochowski

DOI
https://doi.org/10.1038/s41467-022-28074-5
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 14

Abstract

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Transcriptional terminators are generally viewed as hard endpoints for transcribing RNA polymerases. Here, the authors reimagine terminators as transcriptional valves with predictable read through. They engineer and characterize 1780 valves and use them for multiplexed gene regulation.