Sperm Quality Affected by Naturally Occurring Chemical Elements in Bull Seminal Plasma
Filip Tirpák,
Marko Halo,
Marián Tomka,
Tomáš Slanina,
Katarína Tokárová,
Martyna Błaszczyk-Altman,
Lucia Dianová,
Peter Ivanič,
Róbert Kirchner,
Agnieszka Greń,
Norbert Lukáč,
Peter Massányi
Affiliations
Filip Tirpák
AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
Marko Halo
AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
Marián Tomka
Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
Tomáš Slanina
Institute of Applied Biology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
Katarína Tokárová
Institute of Applied Biology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
Martyna Błaszczyk-Altman
Institute of Biology, Pedagogical University of Krakow, Podchorazych 2, 30-084 Krakow, Poland
Lucia Dianová
Institute of Applied Biology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
Peter Ivanič
Slovak Biological Services, Kremnička 2, 974 05 Banská Bystrica, Slovakia
Róbert Kirchner
Institute of Applied Biology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
Agnieszka Greń
Institute of Biology, Pedagogical University of Krakow, Podchorazych 2, 30-084 Krakow, Poland
Norbert Lukáč
Institute of Applied Biology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
Peter Massányi
Institute of Applied Biology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
This study monitored the chemical and biochemical composition of bovine seminal plasma (SP). Freshly ejaculated semen (n = 20) was aliquoted into two parts. The first aliquot was immediately assessed to determine the sperm motion parameters. Another motility measurement was performed following an hour-long co-incubation of spermatozoa with SP at 6 °C. The other aliquot was processed to obtain the SP. Seminal plasma underwent the analyses of chemical composition and quantification of selected proteins, lipids and RedOx markers. Determined concentrations of observed parameters served as input data to correlation analyses where associations between micro and macro elements and RedOx markers were observed. Significant correlations of total oxidant status were found with the content of Cu and Mg. Further significant correlations of glutathione peroxidase were detected in relation to Fe and Hg. Furthermore, associations of chemical elements and RedOx markers and spermatozoa quality parameters were monitored. The most notable correlations indicate beneficial effects of seminal Fe on motility and Mg on velocity and viability of spermatozoa. On the contrary, negative correlations were registered between Zn and sperm velocity and seminal cholesterol content and motility. Our findings imply that seminal plasma has a prospective to be developed as the potential biomarker of bull reproductive health.
