Human lung epithelial cell A549 proteome data after treatment with titanium dioxide and carbon black
Ngoc Q. Vuong,
Patrick Goegan,
Susantha Mohottalage,
Dalibor Breznan,
Marianne Ariganello,
Andrew Williams,
Fred Elisma,
Subramanian Karthikeyan,
Renaud Vincent,
Premkumari Kumarathasan
Affiliations
Ngoc Q. Vuong
Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9; Department of Biochemistry, Faculty of Science, University of Ottawa, Ottawa, ON, Canada K1H 8M5
Patrick Goegan
Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9
Susantha Mohottalage
Analytical Biochemistry and Proteomics, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9
Dalibor Breznan
Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9
Marianne Ariganello
Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9
Andrew Williams
Biostatistics Section, Population Studies Division, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9
Fred Elisma
Analytical Biochemistry and Proteomics, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9
Subramanian Karthikeyan
Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9
Renaud Vincent
Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9; Department of Biochemistry, Faculty of Science, University of Ottawa, Ottawa, ON, Canada K1H 8M5
Premkumari Kumarathasan
Analytical Biochemistry and Proteomics, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9; Interdisciplinary School of Health Sciences, Faculty of Health Sciences, University of Ottawa, Ottawa, ON, Canada K1H 8M5; Corresponding author.
Here, we have described the dataset relevant to the A549 cellular proteome changes after exposure to either titanium dioxide or carbon black particles as compared to the non-exposed controls, “Proteomic changes in human lung epithelial cells (A549) in response to carbon black and titanium dioxide exposures” (Vuong et al., 2016) [1]. Detailed methodologies on the separation of cellular proteins by 2D-GE and the subsequent mass spectrometry analyses using MALDI-TOF-TOF-MS are documented. Particle exposure-specific protein expression changes were measured via 2D-GE spot volume analysis. Protein identification was done by querying mass spectrometry data against SwissProt and RefSeq protein databases using Mascot search engine. Two-way ANOVA analysis data provided information on statistically significant A549 protein expression changes associated with particle exposures. Keywords: Titanium dioxide, Carbon black, A549, Proteomics, 2D-GE, MALDI-TOF-TOF-MS, Toxicity
