Exposure of larval pinto abalone to ocean acidification and warming negatively impacts survival, settlement, and size

Abstract

Abstract Pinto abalone (Haliotis kamtschatkana), the only abalone species native to Washington, declined by 97% in the state from 1992 to 2017. Their decline is a loss for indigenous tribes, recreational divers, and the health of subtidal rocky reefs and kelp beds. Current restoration actions are facing threats of ocean acidification and warming in the northeast Pacific. This research aims to deepen our understanding of the tolerance and physiological flexibility of early life history stages of pinto abalone and inform hatchery practices under future climate change scenarios. We conducted an experiment to test how seawater pH and temperature stress impact abalone larvae. We exposed abalone post-fertilization to elevated temperature and reduced seawater pH for ten days spanning their larval development period: (1) 7.95pH/14°C (ambient), (2) 7.60pH/14°C, (3) 7.95pH/18°C, and (4) 7.60pH/18°C. Abalone in the ambient treatment had the best survival, those in the 7.60pH/18°C treatment had the worst survival, and those in the two single-stressor treatments had survival in between. Among the surviving larvae, pH was the dominant stressor influencing settlement success, with higher settlement rates under ambient pH treatments at both temperatures. pH also had a stronger effect than temperature on shell length. The information gleaned from this study is essential for optimizing future restoration aquaculture for pinto abalone and determining their ideal habitat and potential geographic range.