3D structure and in situ arrangements of CatSper channel in the sperm flagellum

Yanhe Zhao; Huafeng Wang; Caroline Wiesehoefer; Naman B. Shah; Evan Reetz; Jae Yeon Hwang; Xiaofang Huang; Tse-en Wang; Polina V. Lishko; Karen M. Davies; Gunther Wennemuth; Daniela Nicastro; Jean-Ju Chung

doi:10.1038/s41467-022-31050-8

Nature Communications (Jun 2022)

3D structure and in situ arrangements of CatSper channel in the sperm flagellum

Yanhe Zhao,
Huafeng Wang,
Caroline Wiesehoefer,
Naman B. Shah,
Evan Reetz,
Jae Yeon Hwang,
Xiaofang Huang,
Tse-en Wang,
Polina V. Lishko,
Karen M. Davies,
Gunther Wennemuth,
Daniela Nicastro,
Jean-Ju Chung

Affiliations

Yanhe Zhao: Department of Cell Biology, University of Texas Southwestern Medical Center
Huafeng Wang: Department of Cellular and Molecular Physiology, Yale School of Medicine
Caroline Wiesehoefer: Department of Anatomy, University of Duisburg-Essen, Medical Faculty
Naman B. Shah: Department of Molecular and Cell Biology, University of California
Evan Reetz: Department of Cell Biology, University of Texas Southwestern Medical Center
Jae Yeon Hwang: Department of Cellular and Molecular Physiology, Yale School of Medicine
Xiaofang Huang: Department of Cellular and Molecular Physiology, Yale School of Medicine
Tse-en Wang: Department of Cellular and Molecular Physiology, Yale School of Medicine
Polina V. Lishko: Department of Molecular and Cell Biology, University of California
Karen M. Davies: Department of Molecular and Cell Biology, University of California
Gunther Wennemuth: Department of Anatomy, University of Duisburg-Essen, Medical Faculty
Daniela Nicastro: Department of Cell Biology, University of Texas Southwestern Medical Center
Jean-Ju Chung: Department of Cellular and Molecular Physiology, Yale School of Medicine

DOI: https://doi.org/10.1038/s41467-022-31050-8
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

Read online

Sperm motility and male fertility requires function of the CatSper calcium channels. Here, using cryo-electron tomography, authors visualize the native in-cell 3D structure and higher-order organization of the CatSper as long zigzag rows along the sperm tail.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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