Molecular Marker-Assisted Selection for Frost Tolerance in a Diallel Population of Potato
Wei Tu,
Jingcai Li,
Jianke Dong,
Jianghai Wu,
Haibo Wang,
Yingtao Zuo,
Xingkui Cai,
Botao Song
Affiliations
Wei Tu
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
Jingcai Li
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang 438000, China
Jianke Dong
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
Jianghai Wu
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
Haibo Wang
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
Yingtao Zuo
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
Xingkui Cai
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
Botao Song
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
A multi-parental population is an innovative tool for mapping large numbers of loci and genetic modifications, particularly where they have been used for breeding and pre-breeding in crops. Frost injury is an environmental stress factor that greatly affects the growth, development, production efficiency, and geographical distribution of crops. No reported study has focused on genetic mapping and molecular marker development using diallel populations of potatoes. In this study, 23 successful cross combinations, obtained by a half diallel cross among 16 parents, including eight frost-tolerant advanced breeding lines and eight cultivars, were used to map the genetic loci for frost tolerance and to create a molecular marker-assisted selection (MAS) system. Three candidate regions related to frost tolerance on chromosomes II, V, and IX were mapped by bulked segregant analysis (BSA). Furthermore, six SNP markers associated with frost tolerance from candidate regions were developed and validated. Above all, a MAS system for the frost tolerance screening of early breeding offspring was established. This study highlights the practical advantages of applying diallel populations to broaden and improve frost-tolerant germplasm resources.
