Analysis of the Genetic Structure of Slovak White Shorthaired Goat breed using CSN1S1 gene

Abstract

Milk is one of the basic sources of nutrition. With the increasing incidence of allergies and the spread of knowledge about a healthy lifestyle, consumers are changing their eating habits, which partly leads to the reintroduction of goat's milk into the diet. This study aimed to evaluate and describe the genotypic and allelic structure of Slovak White Shorthaired goats for the CSN1S1 gene. For genotyping, PCR, AS-PCR and PIRA-PCR methods. The He and FIS were used for the description of the diversity of the population. Alleles E and F were the most frequent 0.3710 and 0.2944 respectively followed by B (0.1492) and A (0.1169). The less abundant allele was N with an occurrence of 0.0685. The most abundant genotypes were EF (0.2339), BE (0.1693) AN and EE with an occurrence of 0.1129 both. The White Shorthaired goat breed exhibits high values of heterozygosity (0.7661), polymorphism information content(0.6921), the effective number of alleles (3.7736) and level of possible variability realization (74.1%) for the CSN1S1 gene. The inbreeding level of the population represents a negative value (-0.0355), which indicates enough heterozygotes in the population.

Keywords

• white shorthaired goat
• milk
• csn1s1
• genetic structure