Genome-wide identification of microRNAs involved in the somatic embryogenesis of Eucalyptus

Abstract

AbstractMicroRNAs (miRNAs) are small noncoding RNAs (18–24 nt) and function in many biological processes in plants. Although Eucalyptus trees are widely planted across the world, our understanding of the miRNA regulation in the somatic embryogenesis (SE) of Eucalyptus is still poor. Here we reported, for the first time, the miRNA profiles of differentiated and dedifferentiated tissues of two Eucalyptus species and identified miRNAs involved in SE of Eucalyptus. Stem and tissue culture–induced callus were obtained from the subculture seedlings of E. camaldulensisE. grandis x urophyllanE. camaldulensisE. grandis x urophyllaE. camaldulensisARF18GRF6ATHB15/HD-ZIPSPL1e.g.E. camaldulensis