Oral <i>Staphylococcus</i> Species and MRSA Strains in Patients with Orofacial Clefts Undergoing Surgical Rehabilitation Diagnosed by MALDI-TOF MS
Mateus Cardoso Oliveira,
Marcelo Fabiano Gomes Boriollo,
Angélica Cristina de Souza,
Thaísla Andrielle da Silva,
Jeferson Júnior da Silva,
Karina Teixeira Magalhães-Guedes,
Carlos Tadeu dos Santos Dias,
Wagner Luís de Carvalho Bernardo,
José Francisco Höfling,
Cristina Paiva de Sousa
Affiliations
Mateus Cardoso Oliveira
Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba 13414-903, SP, Brazil
Marcelo Fabiano Gomes Boriollo
Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba 13414-903, SP, Brazil
Angélica Cristina de Souza
Department of Biology, Federal University of Lavras (UFLA), s/n Edmir Sá Santos Rotary Interchange, Lavras 37203-202, MG, Brazil
Thaísla Andrielle da Silva
Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba 13414-903, SP, Brazil
Jeferson Júnior da Silva
Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba 13414-903, SP, Brazil
Karina Teixeira Magalhães-Guedes
Department of Bromatological Analysis, Pharmacy Faculty, Federal University of Bahia (UFBA), 147 Barão de Jeremoabo Street, Salvador 40170-115, BA, Brazil
Carlos Tadeu dos Santos Dias
Department of Exact Sciences, College of Agriculture, University of São Paulo (ESALQ/USP), 11 Pádua Dias Ave, Piracicaba 13418-900, SP, Brazil
Wagner Luís de Carvalho Bernardo
Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba 13414-903, SP, Brazil
José Francisco Höfling
Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas (FOP/UNICAMP), 901 Limeira Ave, Piracicaba 13414-903, SP, Brazil
Cristina Paiva de Sousa
Department of Morphology and Pathology & Biotechnology Graduate Program (PPGBiotec), Center for Biological and Health Sciences (CCBS), Federal University of São Carlos (UFSCar), Km 235 Washington Luís Road, São Carlos 13565-905, SP, Brazil
This study investigated the occurrence and dynamics of oral Staphylococcus species in patients with orofacial clefts undergoing surgical rehabilitation treatment. Patients (n = 59) were statistically stratified and analyzed (age, gender, types of orofacial clefts, surgical history, and types of previous surgical rehabilitation). Salivary samples were obtained between hospitalization and the return to the specialized medical center. Microbiological diagnosis was performed by classical methods, and MALDI-TOF MS. MRSA strains (SCCmec type II, III, and IV) were characterized by the Decision Tree method. A total of 33 (55.9%) patients showed oral staphylococcal colonization in one, two, or three sampling steps. A high prevalence has been reported for S. aureus (including HA-, MRSA and CA-MRSA), followed by S. saprophyticus, S. epidermidis, S. sciuri, S. haemolyticus, S. lentus, S. arlettae, and S. warneri. The dynamics of oral colonization throughout surgical treatment and medical follow-up may be influenced by (i) imbalances in staphylococcal maintenance, (ii) efficiency of surgical asepsis or break of the aseptic chain, (iii) staphylococcal neocolonization in newly rehabilitated anatomical oral sites, and (iv) total or partial maintenance of staphylococcal species. The highly frequent clinical periodicity in specialized medical and dental centers may contribute to the acquisition of MRSA in these patients.
