Leaf Proteome Response to Drought Stress and Antioxidant Potential in Tomato (<i>Solanum lycopersicum</i> L.)
Gyanendra Kumar Rai,
Abida Parveen,
Gayatri Jamwal,
Umer Basu,
Ranjeet Ranjan Kumar,
Pradeep K. Rai,
Jag Paul Sharma,
Adel I. Alalawy,
Mohammed A. Al-Duais,
Mohammad Anwar Hossain,
Muhammad Habib ur Rahman,
Ali Raza,
Subhan Danish,
Mohamed I. Sakran
Affiliations
Gyanendra Kumar Rai
School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu 180009, India
Abida Parveen
School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu 180009, India
Gayatri Jamwal
School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu 180009, India
Umer Basu
Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu 180009, India
Ranjeet Ranjan Kumar
Division of Biochemistry, Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India
Pradeep K. Rai
Advance Center for Horticulture Research, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Udheywala, Jammu 180018, India
Jag Paul Sharma
Directorate of Research, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (SKUAST-J), Jammu 180009, India
Adel I. Alalawy
Department of Biochemistry, Faculty of Science, University of Tabuk, Tabuk 47512, Saudi Arabia
Mohammed A. Al-Duais
Department of Biochemistry, Faculty of Science, University of Tabuk, Tabuk 47512, Saudi Arabia
Mohammad Anwar Hossain
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
Muhammad Habib ur Rahman
Crop Science, Institute of Crop Science and Resource Conservation (INRES), University Bonn, 53115 Bonn, Germany
Ali Raza
Fujian Provincial Key Laboratory of Crop Molecular and Cell Biology, Oil Crops Research Institute, Center of Legume Crop Genetics and Systems Biology/College of Agriculture, Fujian Agriculture and Forestry University (FAFU), Fuzhou 350002, China
Subhan Danish
Department of Soil Science, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan 60800, Pakistan
Mohamed I. Sakran
Department of Biochemistry, Faculty of Science, University of Tabuk, Tabuk 47512, Saudi Arabia
Advances in proteome research have opened the gateway to understanding numerous metabolic pathways and fundamental mechanisms involved in abiotic stress tolerance. In the present study, the antioxidant capacity of four tomato genotypes i.e., Kashi Amrit, Kashi Anupam, EC-317-6-1, and WIR-4360 was determined under drought stress to ascertain the scavenging potential for reactive oxygen species (ROS). A significant increase in the superoxide dismutase (SOD), Ascorbate peroxidase (APX), and catalase (CAT) activities in all the four genotypes under drought stress was observed, which seemed to be associated with a protective role against ROS (p Solanum pimpinellifolium) and Kashi Amrit (Solanum lycopersicum) grown under irrigated, drought, and re-watering conditions. To reveal the protein network regulated under these conditions, two-dimensional gel electrophoresis was employed to identify and quantify the number of proteins in drought-sensitive (Kashi Amrit) and tolerant (EC-317-6-1) genotypes. Matrix-assisted laser desorption/ionization-time of flight analysis (MALDI-TOF) revealed a total of 453 spots after fine-tuning factors i.e., smoothness, saliency, and minimum area that responded to drought. Out of 453 total spots, 93 spots were identified in Kashi Amrit and 154 in EC-317-6-1 under irrigated conditions, whereas 4 spots were identified in Kashi Amrit and 77 spots in EC-317-6-1 under drought conditions. Furthermore, differentially expressed proteins were distinguished according to the fold change of their expression. Information provided in this report will be useful for the selection of proteins or genes in analyzing or improving drought tolerance in tomato cultivars. These findings may assist in the construction of a complete proteome database encompassing various divergent species which could be a valuable source for the improvement of crops under drought-stress conditions in the future.
