EXPERIMENTAL RATIONALE OF ENDODONTIC THERAPY OF CHRONIC FORMS OF PULPITIS AND PERIODONTITIS USING PHOTO-ACTIVATED DISINFECTION AND ULTRASOUND TREATMENT

Abstract

Aim. Laboratory rationale of application of a complex effect of ultrasound treatment and photo-activated disinfection in experiments in vitro using strains of anaerobic and microaerophilic microorganisms isolated from patients with chronic forms of pulpitis and periodontitis. Materials and methods. Microbiologic study of root canal was carried out using a bacteriologic method (2 species of obligatory-anaerobic and 2 - microaerophilic streptococci). Evaluation of the effect of ultrasound treatment in a complex with a system of photo-activated disinfection on microflora of root canal was carried out using a bioreactor. Study of biofilms of the root canal system of a tooth in patients with chronic forms of pulpitis (52 individuals) and periodontitis (16 individuals) were carried out using scanning electron microscopy. Results. The presence of an ample microbial biofilm in the ostiums of dentinal canaliculi and obturation of dentinal canaliculi in certain cases was established. Features of formation of growth curves for bacterial populations of oral microflora (Porphyromonas gingivalis, Fusobacterium nucleatum, Streptococcus sanguinis, Streptococcus mutans) were described in the experimental part of the study as a result of a complex effect of photodynamic activation and ultrasound compared with control lacking those. Conclusion. The data obtained allow to conclude that complex effect significantly suppresses the growth or microbial populations of oral bacteria.

Keywords

• chronic pulpitis
• root channels
• microbial biofilm
• endodontic therapy
• ultrasound irrigation
• photodynamic therapy
• bioreactor
• antimicrobial activity in vitro