Role of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission

Li Yang; Shifeng Nian; Guozhen Zhang; Edward Sharman; Hui Miao; Xuepeng Zhang; Xiaofeng Chen; Yi Luo; Jun Jiang

doi:10.1038/s41598-019-47660-0

Scientific Reports (Aug 2019)

Role of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission

Li Yang,
Shifeng Nian,
Guozhen Zhang,
Edward Sharman,
Hui Miao,
Xuepeng Zhang,
Xiaofeng Chen,
Yi Luo,
Jun Jiang

Affiliations

Li Yang: Institutes of Physical Science and Information Technology, Anhui University
Shifeng Nian: Hefei National Laboratory for Physical Sciences at the Microscale, iChEM (Collaborative Innovation Centre of Chemistry for Energy Materials), CAS Centre for Excellence in Nanoscience, Department of Chemistry and Materials Science, University of Science and Technology of China
Guozhen Zhang: Hefei National Laboratory for Physical Sciences at the Microscale, iChEM (Collaborative Innovation Centre of Chemistry for Energy Materials), CAS Centre for Excellence in Nanoscience, Department of Chemistry and Materials Science, University of Science and Technology of China
Edward Sharman: Department of Neurology, University of California, Irvine
Hui Miao: Hefei National Laboratory for Physical Sciences at the Microscale, iChEM (Collaborative Innovation Centre of Chemistry for Energy Materials), CAS Centre for Excellence in Nanoscience, Department of Chemistry and Materials Science, University of Science and Technology of China
Xuepeng Zhang: Hefei National Laboratory for Physical Sciences at the Microscale, iChEM (Collaborative Innovation Centre of Chemistry for Energy Materials), CAS Centre for Excellence in Nanoscience, Department of Chemistry and Materials Science, University of Science and Technology of China
Xiaofeng Chen: Department of Environmental Science and Engineering, College of Life and Environmental Science, Shanghai Normal University
Yi Luo: Hefei National Laboratory for Physical Sciences at the Microscale, iChEM (Collaborative Innovation Centre of Chemistry for Energy Materials), CAS Centre for Excellence in Nanoscience, Department of Chemistry and Materials Science, University of Science and Technology of China
Jun Jiang: Hefei National Laboratory for Physical Sciences at the Microscale, iChEM (Collaborative Innovation Centre of Chemistry for Energy Materials), CAS Centre for Excellence in Nanoscience, Department of Chemistry and Materials Science, University of Science and Technology of China

DOI: https://doi.org/10.1038/s41598-019-47660-0
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 7

Abstract

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Abstract The fluorescence emission from green fluorescent protein (GFP) is known to be heavily influenced by hydrogen bonding between the core fluorophore and the surrounding side chains or water molecules. Yet how to utilize this feature for modulating the fluorescence of GFP chromophore or GFP-like fluorophore still remains elusive. Here we present theoretical calculations to predict how hydrogen bonding could influence the excited states of the GFP-like fluorophores. These studies provide both a new perspective for understanding the photophysical properties of GFP as well as a solid basis for the rational design of GFP-based fluorophores.

Published in Scientific Reports

ISSN: 2045-2322 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://www.nature.com/srep/

About the journal