Lung cDC1 and cDC2 dendritic cells priming naive CD8+ T cells in situ prior to migration to draining lymph nodes
Youhui Si,
Yihan Wang,
Qiaomu Tian,
Qiang Wang,
Jared M. Pollard,
Pramod K. Srivastava,
Aaron P. Esser-Kahn,
Joel H. Collier,
Anne I. Sperling,
Anita S. Chong
Affiliations
Youhui Si
National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Department of Surgery, The University of Chicago, Chicago, IL 60637, USA; Corresponding author
Yihan Wang
Department of Surgery, The University of Chicago, Chicago, IL 60637, USA
Qiaomu Tian
Department of Surgery, The University of Chicago, Chicago, IL 60637, USA
Qiang Wang
Department of Surgery, The University of Chicago, Chicago, IL 60637, USA
Jared M. Pollard
Department of Surgery, The University of Chicago, Chicago, IL 60637, USA
Pramod K. Srivastava
Department of Immunology and Carole and Ray Neag Comprehensive Cancer Center, University of Connecticut School of Medicine, Farmington, CT 06032, USA
Aaron P. Esser-Kahn
Pritzker School of Molecular Engineering, The University of Chicago, Chicago, IL 60637, USA
Joel H. Collier
Department of Biomedical Engineering, Duke University, Durham, NC 27710, USA
Anne I. Sperling
Department of Medicine, Pulmonary and Critical Care, University of Virginia, Charlottesville, VA 22908, USA
Anita S. Chong
Department of Surgery, The University of Chicago, Chicago, IL 60637, USA; Corresponding author
Summary: The current paradigm indicates that naive T cells are primed in secondary lymphoid organs. Here, we present evidence that intranasal administration of peptide antigens appended to nanofibers primes naive CD8+ T cells in the lung independently and prior to priming in the draining mediastinal lymph node (MLN). Notably, comparable accumulation and transcriptomic responses of CD8+ T cells in lung and MLN are observed in both Batf3KO and wild-type (WT) mice, indicating that, while cDC1 dendritic cells (DCs) are the major subset for cross-presentation, cDC2 DCs alone are capable of cross-priming CD8+ T cells both in the lung and draining MLN. Transcription analyses reveal distinct transcriptional responses in lung cDC1 and cDC2 to intranasal nanofiber immunization. However, both DC subsets acquire shared transcriptional responses upon migration into the lymph node, thus uncovering a stepwise activation process of cDC1 and cDC2 toward their ability to cross-prime effector and functional memory CD8+ T cell responses.
