Generic Workflow of a Highly Effective and Easy Anther Culture Method for Both Japonica and Indica Rice
Guimei Guo,
Shisen Liu,
Shuwei Zhang,
Linian Yang,
Yingjie Zong,
Nigel G. Halford,
Ting He,
Runhong Gao,
Zhenzhu Guo,
Longhua Zhou,
Chenghong Liu,
Shujun Wu,
Zhiwei Chen
Affiliations
Guimei Guo
Shanghai Key Laboratory of Agricultural Genetics and Breeding (21DZ2271900), Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms of Ministry of Agriculture and Rural Affairs (Shanghai), Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Shisen Liu
Shanghai Key Laboratory of Agricultural Genetics and Breeding (21DZ2271900), Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms of Ministry of Agriculture and Rural Affairs (Shanghai), Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Shuwei Zhang
Shanghai Key Laboratory of Agricultural Genetics and Breeding (21DZ2271900), Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms of Ministry of Agriculture and Rural Affairs (Shanghai), Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Linian Yang
Shanghai Key Laboratory of Agricultural Genetics and Breeding (21DZ2271900), Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms of Ministry of Agriculture and Rural Affairs (Shanghai), Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Yingjie Zong
Shanghai Key Laboratory of Agricultural Genetics and Breeding (21DZ2271900), Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms of Ministry of Agriculture and Rural Affairs (Shanghai), Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Nigel G. Halford
Rothamsted Research, Harpenden AL5 2JQ, UK
Ting He
Shanghai Key Laboratory of Agricultural Genetics and Breeding (21DZ2271900), Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms of Ministry of Agriculture and Rural Affairs (Shanghai), Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Runhong Gao
Shanghai Key Laboratory of Agricultural Genetics and Breeding (21DZ2271900), Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms of Ministry of Agriculture and Rural Affairs (Shanghai), Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Zhenzhu Guo
Shanghai Key Laboratory of Agricultural Genetics and Breeding (21DZ2271900), Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms of Ministry of Agriculture and Rural Affairs (Shanghai), Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Longhua Zhou
Shanghai Key Laboratory of Agricultural Genetics and Breeding (21DZ2271900), Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms of Ministry of Agriculture and Rural Affairs (Shanghai), Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Chenghong Liu
Shanghai Key Laboratory of Agricultural Genetics and Breeding (21DZ2271900), Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms of Ministry of Agriculture and Rural Affairs (Shanghai), Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
Shujun Wu
Crop Breeding & Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Zhiwei Chen
Shanghai Key Laboratory of Agricultural Genetics and Breeding (21DZ2271900), Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms of Ministry of Agriculture and Rural Affairs (Shanghai), Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
As one of the most important staple crops in the world, rice plays a pivotal role in world food security. The creation of doubled haploids based on anther culture is an important technology for rice breeding. However, at present, rice anther culture technology still faces many problems, such as genotype dependency, especially genotypes of indica rice. In this study, fifteen rice genotypes, including twelve japonica rice genotypes and three indica rice genotypes, were randomly selected and used to study anther culture by using a modified M8 medium. The results showed that the total callus induction rates of these different rice genotypes ranged from 0.81 to 13.95%, with an average of 6.64%, while the callus induction rates calculated for the top ten highest callus inductions for each rice genotype ranged from 2.75 to 17.00%, with an average of 10.56%. There were varying gaps between the total callus induction rates and the callus induction rates in these different rice genotypes. The fact that the gaps for some rice genotypes were relatively large indicated that standard tiller or anther collection was not applicable to all rice genotypes and that there was still a lot of room for improvement in the callus induction rate of some rice genotypes through optimization of the sampling method. The plantlet regeneration rates ranged from 12.55 to 456.54%, with an average of 200.10%. Although there were many albinos from anther culture for some rice genotypes, these would still meet the requirement if the rice genotypes had higher callus induction rates or regeneration rates. The percentages of seed setting of regenerated green seedlings ranged from 14% to 84%, with an average of 48.73%. Genetic diversity analysis showed that the genetic background of these different rice genotypes was representative, and the phylogenetic tree and Principal Component Analysis (PCA) divided them into indica and japonica types. Therefore, in this study, an anther culture method suitable for both indica and japonica rice genotypes was established, which could improve doubled haploid breeding in rice.
