Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL

Elena N. Sigida; Ibrahim M. Ibrahim; Maxim S. Kokoulin; Hussein H. Abulreesh; Khaled Elbanna; Svetlana A. Konnova; Yulia P. Fedonenko

doi:10.3390/md19090508

Marine Drugs (Sep 2021)

Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL

Elena N. Sigida,
Ibrahim M. Ibrahim,
Maxim S. Kokoulin,
Hussein H. Abulreesh,
Khaled Elbanna,
Svetlana A. Konnova,
Yulia P. Fedonenko

Affiliations

Elena N. Sigida: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049 Saratov, Russia
Ibrahim M. Ibrahim: N. G. Chernyshevsky Saratov State University, 83 Ulitsa Astrakhanskaya, 410012 Saratov, Russia
Maxim S. Kokoulin: G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, 159 Prospekt 100 let Vladivostoku, 690022 Vladivostok, Russia
Hussein H. Abulreesh: Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah 21955, Saudi Arabia
Khaled Elbanna: Department of Agricultural Microbiology, Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt
Svetlana A. Konnova: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049 Saratov, Russia
Yulia P. Fedonenko: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049 Saratov, Russia

DOI: https://doi.org/10.3390/md19090508
Journal volume & issue: Vol. 19, no. 9
p. 508

Abstract

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The moderately halophilic strain Salinivibrio sp. EG9S8QL was isolated among 11 halophilic strains from saline mud (Emisal Salt Company, Lake Qarun, Fayoum, Egypt). The lipopolysaccharide was extracted from dried cells of Salinivibrio sp. EG9S8QL by the phenol–water procedure. The OPS was obtained by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with 1H and 13C NMR spectroscopy, including 1H,1H COSY, TOCSY, ROESY, 1H,13C HSQC, and HMBC experiments. The OPS was found to be composed of linear tetrasaccharide repeating units of the following structure: →2)-β-Manp4Lac-(1→3)-α-ManpNAc-(1→3)-β-Rhap-(1→4)-α-GlcpNAc-(1→, where Manp4Lac is 4-O-[1-carboxyethyl]mannose.

Published in Marine Drugs

ISSN: 1660-3397 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: http://www.mdpi.com/journal/marinedrugs

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