Structure of the 4-O-[1-Carboxyethyl]-<span style="font-variant: small-caps">d</span>-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium <i>Salinivibrio</i> sp. EG9S8QL
Elena N. Sigida,
Ibrahim M. Ibrahim,
Maxim S. Kokoulin,
Hussein H. Abulreesh,
Khaled Elbanna,
Svetlana A. Konnova,
Yulia P. Fedonenko
Affiliations
Elena N. Sigida
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049 Saratov, Russia
Ibrahim M. Ibrahim
N. G. Chernyshevsky Saratov State University, 83 Ulitsa Astrakhanskaya, 410012 Saratov, Russia
Maxim S. Kokoulin
G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, 159 Prospekt 100 let Vladivostoku, 690022 Vladivostok, Russia
Hussein H. Abulreesh
Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah 21955, Saudi Arabia
Khaled Elbanna
Department of Agricultural Microbiology, Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt
Svetlana A. Konnova
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049 Saratov, Russia
Yulia P. Fedonenko
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049 Saratov, Russia
The moderately halophilic strain Salinivibrio sp. EG9S8QL was isolated among 11 halophilic strains from saline mud (Emisal Salt Company, Lake Qarun, Fayoum, Egypt). The lipopolysaccharide was extracted from dried cells of Salinivibrio sp. EG9S8QL by the phenol–water procedure. The OPS was obtained by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with 1H and 13C NMR spectroscopy, including 1H,1H COSY, TOCSY, ROESY, 1H,13C HSQC, and HMBC experiments. The OPS was found to be composed of linear tetrasaccharide repeating units of the following structure: →2)-β-Manp4Lac-(1→3)-α-ManpNAc-(1→3)-β-Rhap-(1→4)-α-GlcpNAc-(1→, where Manp4Lac is 4-O-[1-carboxyethyl]mannose.
