Nature Communications (May 2022)

Characterization of RNA content in individual phase-separated coacervate microdroplets

  • Damian Wollny,
  • Benjamin Vernot,
  • Jie Wang,
  • Maria Hondele,
  • Aram Safrastyan,
  • Franziska Aron,
  • Julia Micheel,
  • Zhisong He,
  • Anthony Hyman,
  • Karsten Weis,
  • J. Gray Camp,
  • T.‐Y. Dora Tang,
  • Barbara Treutlein

DOI
https://doi.org/10.1038/s41467-022-30158-1
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 9

Abstract

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Here, the authors demonstrate that single cell RNA sequencing technology can be leveraged to characterize RNA content of individual membrane-free condensates formed by liquid-liquid phase separation processes such as coacervation.