Sensitive CG-ICA based on heterologous coating antigen and mAb prepared with carbons-linker immunogen

Abstract

Amantadine (AMD) is harmful to human health and has been forbidden as an antiviral drug in the livestock and poultry breeding. Thus, a rapid and sensitive detection method for AMD should be established. In this research, we have designed three kinds of haptens and then prepared three kinds of immunogens and coating antigens. A highly sensitive colloidal gold immunochromatographic assay (CG-ICA) for AMD detection by using heterologous coating antigen and mAb prepared with immunogen adding five carbons linker has been established. Under optimal conditions, the cut-off value of the CG-ICA was 2.5 ng/mL by naked eyes. The CG-ICA presented a good signal response to AMD in the concentration range of 0.1–2.5 ng/mL with low LOD at 0.077 ng/mL. These results indicated that the immunogen with carbon linker may obtain high-affinity antibody while the heterologous coating antigen may improve the sensitivity of ICA.

Keywords

• amantadine
• monoclonal antibody
• heterologous coating antigen
• colloidal gold immunochromatographic assay
• chicken