Production of diseases - free plant materials of grapevine from phytoplasma in vitro

Abstract

Heat treatment was used as a method of improving the health of grape plants from the phytoplasma. The Binder KBWF 240 growth cabinet was used for thermotherapy, in which plants of the Tsitronny Magaracha variety were cultivated in vitro under simulated conditions with a treatment period of 14 days. The results of repeated testing of samples showed the efficacy of treatment of this grape disease: out of 13 samples of the Tsitronny Magaracha variety, only one revealed the latent form of phytoplasma blackening of grapevine wood Bois noir. To improve the health of grape plants obtained in vitro culture, it is advisable to use the developed technological operations with thermotherapy in the growth cabinet, which allows to rid the planting material of the latent form of phytoplasma associated with blackening of grapevine wood Bois noir.