Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults

Katerina Vlahos; Koula Sourris; Robyn Mayberry; Penelope McDonald; Freya F. Bruveris; Jacqueline V. Schiesser; Kiymet Bozaoglu; Paul J. Lockhart; Edouard G. Stanley; Andrew G. Elefanty

Stem Cell Research (Jan 2019)

Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults

Katerina Vlahos,
Koula Sourris,
Robyn Mayberry,
Penelope McDonald,
Freya F. Bruveris,
Jacqueline V. Schiesser,
Kiymet Bozaoglu,
Paul J. Lockhart,
Edouard G. Stanley,
Andrew G. Elefanty

Affiliations

Katerina Vlahos: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Corresponding author.
Koula Sourris: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia
Robyn Mayberry: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia
Penelope McDonald: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia
Freya F. Bruveris: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia
Jacqueline V. Schiesser: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia
Kiymet Bozaoglu: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia
Paul J. Lockhart: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia
Edouard G. Stanley: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia
Andrew G. Elefanty: Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia

Journal volume & issue: Vol. 34

Abstract

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Abstract: We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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