Characterizing interaction between the juxtamembrane region of the single transmembrane protein and membrane using chemically synthesized peptides

Abstract

Summary: In membrane proteins, a transmembrane region and a juxtamembrane region play important roles in its function. Here, we present a protocol for characterizing membrane protein dynamics between the juxtamembrane region of the single transmembrane protein and acidic membrane. We describe steps for solid-phase peptide synthesis, peptide purification, and labeling. We then detail reconstitution of the transmembrane peptide into lipid bilayers and its evaluation and structural analysis.For complete details on the use and execution of this protocol, please refer to Prasada Rao et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Biophysics
• Protein expression and purification
• Chemistry