Biodegradation of Cellulosic Wastes and Deinking of Colored Paper with Isolated Novel Cellulolytic Bacteria

Abstract

Biofuels are the cheapest source of energy, and the continuous decline of traditional sources of energy with the increasing population leads to looking for alternatives to reduce the consumption of traditional sources of energy. Bioethanol production from lignocellulosic wastes and cellulosic wastes is not a new approach for fuel production but a cheap and accessible way for the production of fuel. Bacillus is one of the major species that can act as a source of diversified enzymes. In this study, it was emphasized on screening and isolation of a novel, characterization, and best catalytic action on both celluloses and proteins in the presence of different carbon and nitrogen sources. It was observed the effective catalytic breakdown of cellulose with the crude enzyme to glucose allowed fur for fermentation with Saccharomyces, ultimately leading to the generation of alcohol. The study aims to isolate the microbes that can produce cellulases and enzymes and could be used for biodegradation to produce ethanol in the reaction. The maximum enzyme activity was achieved at 3.112 UI with optimized pH and temperature, and the maximum conversion of sugars into alcohol was about 70% in the newspaper, cartons, colored paper, and disposable paper cups. An essential observation was the decolorization of the origami craft paper within 24 hours. The study was involved in enhancing the maximum Enzyme activity of cellulases from different cellulosic raw materials. Hence, it was achieved by JCB strain, optimization of pH, temperature, and acids for the biodegradation. The presence of peaks at 3200 and 2900 was a confirmation of ethanol bonds in the biodegradation reaction mixtures.

Keywords

• cellulases, bioethanol, bacillus paramycoides, deinking, endoglucanases