Spożywanie piwa i alkoholu etylowego a poziom wybranych interleukin w osoczu u młodych szczurów Wistar

Abstract

Introduction: Excessive alcohol intake can directly inhibit the immune response. Ethyl alcohol affects changes of plasma cytokine concentrations and in various tissues disturbs the balance between the multifunctional proteins that play a pivotal role in immunological and inflammatory responses. The aim of this study was to evaluate the effect of beer or ethanol consumption on selected plasma cytokine concentrations in young male rats. Material and methods: The experiment was conducted on 30-day old male Wistar rats divided into 4 groups (B2, B6, E2, and E6) and 2 control groups (C2 and C6). Rats from B2 and B6 groups were exposed to beer (10% of ethanol), and those from E2 and E6 groups to 10% ethanol solution for 2 or 6 weeks respectively. The rats had free access to alcohol and lab chow, but water was supplied during the photoperiod light phase. The rats were anesthetised after two or six weeks of the experiment and the blood was sampled by heart puncture and collected into heparinised tubes. Plasma IL-6 and IL-10 concentrations were measured using ELISA kits. Results: Statistical analysis has shown that plasma IL-10 concentration was significantly increased in E6 versus C6 and E2 groups of rats. Decreased plasma IL-6 concentration in E2 in comparison with C2 group of rats was also found. At the same time, significantly higher IL-6 concentration was observed in B6 versus C6 and E6 groups. Our results indicate disruption of cytokine production, which may impair immunological defence and influence proinflammatory reactions.

Keywords

• Cytokines
• Interleukin-6 (IL-6)
• Interleukin-10 (IL-10)
• Beer
• Ethyl alcohol
• Wistar rats