Effect of Crude Polysaccharides from <i>Ecklonia cava</i> Hydrolysate on Cell Proliferation and Differentiation of <i>Hanwoo</i> Muscle Stem Cells for Cultured Meat Production

Abstract

Ecklonia cava, a brown seaweed native to the East Asian coast, is known for its unique composition, including polysaccharides, polyphenols, and phlorotannins. Fucoidan is a sulfated polysaccharide widely used as a functional ingredient in foods. This study obtained crude polysaccharides (ECC_CPS) from E. cava celluclast enzymatic hydrolysate using ethanol precipitation. ECC_CPS increased cell viability during the proliferation of Hanwoo muscle satellite cells (HMSCs). The effect of ECC_CPS on the expression of proliferation-related markers was confirmed as MYF5 and MYOD expression significantly increased, whereas PAX7 expression was maintained. The evaluation of cell migration activity has a major impact on cell proliferation and differentiation, and the cell migration index significantly increased with ECC_CPS treatment (p MYH2, MYH7, and MYOG (p p < 0.01). Therefore, our findings imply that crude polysaccharide obtained from E. cava can be an additive ingredient that enhances the proliferation and differentiation of muscle satellite cells used in the manufacture of cultured meat products.

Keywords

• <i>Ecklonia cava</i>
• polysaccharide
• proliferation
• differentiation
• <i>Hanwoo</i> muscle satellite cells