Airway Epithelial Cultures of Children with Esophageal Atresia as a Model to Study Respiratory Tract Disorders
Henriette H. M. Dreyer,
Eleonora Sofie van Tuyll van Serooskerken,
Lisa W. Rodenburg,
Arnold J. N. Bittermann,
Hubertus G. M. Arets,
Ellen M. B. P. Reuling,
Johannes W. Verweij,
Eric G. Haarman,
David C. van der Zee,
Stefaan H. A. J. Tytgat,
Cornelis K. van der Ent,
Jeffrey M. Beekman,
Gimano D. Amatngalim,
Maud Y. A. Lindeboom
Affiliations
Henriette H. M. Dreyer
Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center, 3508 AB Utrecht, The Netherlands
Eleonora Sofie van Tuyll van Serooskerken
Department of Pediatric Surgery, Wilhelmina Children’s Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands
Lisa W. Rodenburg
Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center, 3508 AB Utrecht, The Netherlands
Arnold J. N. Bittermann
Pediatric Upper Gastrointestinal and Airway Treatment Center, Wilhelmina Children’s Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands
Hubertus G. M. Arets
Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center, 3508 AB Utrecht, The Netherlands
Ellen M. B. P. Reuling
Department of Pediatric Surgery, Wilhelmina Children’s Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands
Johannes W. Verweij
Department of Pediatric Surgery, Wilhelmina Children’s Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands
Eric G. Haarman
Department of Paediatric Pulmonology, Emma Children’s Hospital, Amsterdam UMC, 1105 AZ Amsterdam, The Netherlands
David C. van der Zee
Department of Pediatric Surgery, Wilhelmina Children’s Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands
Stefaan H. A. J. Tytgat
Department of Pediatric Surgery, Wilhelmina Children’s Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands
Cornelis K. van der Ent
Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center, 3508 AB Utrecht, The Netherlands
Jeffrey M. Beekman
Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center, 3508 AB Utrecht, The Netherlands
Gimano D. Amatngalim
Department of Pediatric Pulmonology, Wilhelmina Children’s Hospital, University Medical Center, 3508 AB Utrecht, The Netherlands
Maud Y. A. Lindeboom
Department of Pediatric Surgery, Wilhelmina Children’s Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands
Esophageal atresia (EA) is a rare birth defect in which respiratory tract disorders are a major cause of morbidity. It remains unclear whether respiratory tract disorders are in part caused by alterations in airway epithelial cell functions such as the activity of motile cilia. This can be studied using airway epithelial cell culture models of patients with EA. Therefore, the aim of this study was to evaluate the feasibility to culture and functionally characterize motile cilia function in the differentiated air–liquid interface cultured airway epithelial cells and 3D organoids derived from nasal brushings and bronchoalveolar lavage (BAL) fluid from children with EA. We demonstrate the feasibility of culturing differentiated airway epithelia and organoids of nasal brushings and BAL fluid of children with EA, which display normal motile cilia function. EA patient-derived airway epithelial cultures can be further used to examine whether alterations in epithelial functions contribute to respiratory disorders in EA.
