Capsaicin Decreases Kidney Iron Deposits and Increases Hepcidin Levels in Diabetic Rats with Iron Overload: A Preliminary Study
Marisa López,
Laura Quintero-Macías,
Miguel Huerta,
Alejandrina Rodríguez-Hernández,
Valery Melnikov,
Yolitzy Cárdenas,
Jaime Alberto Bricio-Barrios,
Enrique Sánchez-Pastor,
Armando Gamboa-Domínguez,
Caridad Leal,
Xóchitl Trujillo,
Mónica Ríos-Silva
Affiliations
Marisa López
University Center of Biomedical Research, Universidad de Colima, Av. 25 de Julio #965, Col. Villas San Sebastian, Colima 28045, Mexico
Laura Quintero-Macías
Faculty of Medicine, Universidad de Colima, Av. Universidad #333, Col. Las Víboras, Colima 28040, Mexico
Miguel Huerta
University Center of Biomedical Research, Universidad de Colima, Av. 25 de Julio #965, Col. Villas San Sebastian, Colima 28045, Mexico
Alejandrina Rodríguez-Hernández
Faculty of Medicine, Universidad de Colima, Av. Universidad #333, Col. Las Víboras, Colima 28040, Mexico
Valery Melnikov
Faculty of Medicine, Universidad de Colima, Av. Universidad #333, Col. Las Víboras, Colima 28040, Mexico
Yolitzy Cárdenas
University Center of Biomedical Research, Universidad de Colima, Av. 25 de Julio #965, Col. Villas San Sebastian, Colima 28045, Mexico
Jaime Alberto Bricio-Barrios
Faculty of Medicine, Universidad de Colima, Av. Universidad #333, Col. Las Víboras, Colima 28040, Mexico
Enrique Sánchez-Pastor
University Center of Biomedical Research, Universidad de Colima, Av. 25 de Julio #965, Col. Villas San Sebastian, Colima 28045, Mexico
Armando Gamboa-Domínguez
Belisario Domínguez Sección XVI, Pathology Department, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City 14080, Mexico
Caridad Leal
Centro de Investigaciones Biomédicas de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada No. 800, Col. Independencia, Guadalajara 44340, Mexico
Xóchitl Trujillo
University Center of Biomedical Research, Universidad de Colima, Av. 25 de Julio #965, Col. Villas San Sebastian, Colima 28045, Mexico
Mónica Ríos-Silva
University Center of Biomedical Research, CONACyT-Universidad de Colima, Av. 25 de Julio #965, Col. Villas San Sebastian, Colima 28045, Mexico
Iron overload (IOL) increases the risk of diabetes mellitus (DM). Capsaicin (CAP), an agonist of transient receptor potential vanilloid-1 (TRPV1), reduces the effects of IOL. We evaluated the effects of chronic CAP administration on hepcidin expression, kidney iron deposits, and urinary biomarkers in a male Wistar rat model with IOL and DM (DM-IOL). IOL was induced with oral administration of iron for 12 weeks and DM was induced with streptozotocin. Four groups were studied: Healthy, DM, DM-IOL, and DM-IOL + CAP (1 mg·kg−1·day−1 for 12 weeks). Iron deposits were visualized with Perls tissue staining and a colorimetric assay. Serum hepcidin levels were measured with an enzyme-linked immunosorbent assay. Kidney biomarkers were assayed in 24 h urine samples. In the DM-IOL + CAP group, the total area of iron deposits and the total iron content in kidneys were smaller than those observed in both untreated DM groups. CAP administration significantly increased hepcidin levels in the DM-IOL group. Urinary levels of albumin, cystatin C, and beta-2-microglobulin were similar in all three experimental groups. In conclusion, we showed that in a DM-IOL animal model, CAP reduced renal iron deposits and increased the level of circulating hepcidin.