PLoS ONE (Jan 2022)

Effect of an antenatal diet and lifestyle intervention and maternal BMI on cord blood DNA methylation in infants of overweight and obese women: The LIMIT Randomised Controlled Trial.

  • Jennie Louise,
  • Andrea R Deussen,
  • Berthold Koletzko,
  • Julie Owens,
  • Richard Saffery,
  • Jodie M Dodd

DOI
https://doi.org/10.1371/journal.pone.0269723
Journal volume & issue
Vol. 17, no. 6
p. e0269723

Abstract

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BackgroundTo investigate the effect of an antenatal diet and lifestyle intervention, and maternal pre-pregnancy overweight or obesity, on infant cord blood DNA methylation.MethodsWe measured DNA methylation in 645 cord blood samples from participants in the LIMIT study (an antenatal diet and lifestyle intervention for women with early pregnancy BMI ≥25.0 kg/m2) using the Illumina 450K BeadChip array, and tested for any differential methylation related to the intervention, and to maternal early pregnancy BMI. We also analysed differential methylation in relation to selected candidate genes.ResultsNo CpG sites were significantly differentially methylated in relation to either the diet and lifestyle intervention, or with maternal early pregnancy BMI. There was no significant differential methylation in any of the selected genes related to the intervention, or to maternal BMI.ConclusionWe found no evidence of an effect of either antenatal diet and lifestyle, or of maternal early pregnancy BMI, on cord blood DNA methylation.Clinical trials registrationACTRN12607000161426.