TRIM69 inhibits cataractogenesis by negatively regulating p53
Xianfang Rong,
Jun Rao,
Dan Li,
Qinghe Jing,
Yi Lu,
Yinghong Ji
Affiliations
Xianfang Rong
Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, Shanghai, 200031, China; Eye Institute, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, Shanghai, 200031, China; National Health Commission (NHC) Key Laboratory of Myopia (Fudan University), No. 83 Fenyang Road, Shanghai, 200031, China; Laboratory of Myopia, Chinese Academy of Medical Sciences, No. 83 Fenyang Road, Shanghai, 200031, China; Key Laboratory of Visual Impairment and Restoration of Shanghai, No. 83 Fenyang Road, Shanghai, 200031, China
Jun Rao
Jiangxi Provincial Key Laboratory of Translational Medicine and Oncology, Jiangxi Cancer Hospital, Jiangxi Cancer Center, Nanchang, 330029, China
Dan Li
Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, Shanghai, 200031, China; Eye Institute, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, Shanghai, 200031, China; National Health Commission (NHC) Key Laboratory of Myopia (Fudan University), No. 83 Fenyang Road, Shanghai, 200031, China; Laboratory of Myopia, Chinese Academy of Medical Sciences, No. 83 Fenyang Road, Shanghai, 200031, China; Key Laboratory of Visual Impairment and Restoration of Shanghai, No. 83 Fenyang Road, Shanghai, 200031, China
Qinghe Jing
Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, Shanghai, 200031, China; Eye Institute, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, Shanghai, 200031, China; National Health Commission (NHC) Key Laboratory of Myopia (Fudan University), No. 83 Fenyang Road, Shanghai, 200031, China; Laboratory of Myopia, Chinese Academy of Medical Sciences, No. 83 Fenyang Road, Shanghai, 200031, China; Key Laboratory of Visual Impairment and Restoration of Shanghai, No. 83 Fenyang Road, Shanghai, 200031, China
Yi Lu
Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, Shanghai, 200031, China; Eye Institute, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, Shanghai, 200031, China; National Health Commission (NHC) Key Laboratory of Myopia (Fudan University), No. 83 Fenyang Road, Shanghai, 200031, China; Laboratory of Myopia, Chinese Academy of Medical Sciences, No. 83 Fenyang Road, Shanghai, 200031, China; Key Laboratory of Visual Impairment and Restoration of Shanghai, No. 83 Fenyang Road, Shanghai, 200031, China
Yinghong Ji
Department of Ophthalmology, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, Shanghai, 200031, China; Eye Institute, Eye & ENT Hospital of Fudan University, No. 83 Fenyang Road, Shanghai, 200031, China; National Health Commission (NHC) Key Laboratory of Myopia (Fudan University), No. 83 Fenyang Road, Shanghai, 200031, China; Laboratory of Myopia, Chinese Academy of Medical Sciences, No. 83 Fenyang Road, Shanghai, 200031, China; Key Laboratory of Visual Impairment and Restoration of Shanghai, No. 83 Fenyang Road, Shanghai, 200031, China; Corresponding author. Department of Ophthalmology & Eye Institute, Eye & ENT Hospital of Fudan University, NHC Key Laboratory of Myopia (Fudan University), Laboratory of Myopia, Chinese Academy of Medical Sciences, and Key Laboratory of Visual Impairment and Restoration of Shanghai, No. 83 Fenyang Road, Shanghai, 200031, China.
Ultraviolet B (UVB) irradiation can induce reactive oxygen species (ROS) production and apoptosis in human lens epithelial cells (HLECs), thus leading to the formation of cataracts. We studied the role of tripartite motif 69 (TRIM69) in cataract formation. The expression of TRIM69 protein was down-regulated in both human cataract capsule tissues and HLECs treated with UVB, whereas the expression of p53 protein exhibited an opposite trend. Ectopic expression of TRIM69 in HLECs significantly suppressed UVB-induced apoptosis and ROS production, whereas knockdown of TRIM69 promoted apoptosis and ROS production. TRIM69 can interact with p53 and induce its ubiquitination. The effects of TRIM69 overexpression in UVB-induced cell apoptosis and ROS production was clearly weakened by p53 overexpression, thus suggesting a role for p53 in TRIM69 functions. Furthermore, inhibition of ROS mitigated the effects of UVB irradiation on ROS production, cell apoptosis, forkhead box protein 3a (Foxo3a) phosphorylation, and TRIM69 expression. Additionally, Foxo3a overexpression significantly enhanced TRIM69 promoter activity, whereas Foxo3a knockdown had the opposite effect. In conclusion, we provide the first demonstration that Foxo3a is a potential transcription factor for TRIM69, and TRIM69 induces p53 ubiquitination. These results suggest that the Foxo3a/TRIM69/p53 regulatory network may be involved in cataract formation. Keywords: Cataract, TRIM69, p53, UVB, Foxo3a
