Identification of some viruses of the medicinal plant Physalis alkekengi L

Abstract

Determining the medical properties of Physalis alkekengi L. extracts, extracting active compounds with antibacterial activity and immunostimulatory properties, that is, physalins and flavonoids, requires the development of biotechnology for the creation of virus-free, healthy medicinal plants by microclonal propagation of plants using modern biotechnological approaches. In this article, TMV-Ph strain of tobacco mosaic virus was isolated from medicinal plant Physalis alkekengi L. and its biological, morphological and physical characteristics were studied. TMV- Ph strain was identified to be belong to the Virgaviridae family of the tobamovirus group. As a result of research on this topic, virus disease symptoms were identified in the Physalis alkekengi plant, and methods for isolating and biologically purifying the virus were developed. To achieve this, viral sap prepared from the leaves of infected plants was inoculated into the Nicotiana sylvestris plant to isolate the virus. For biological purification, infectious sap was prepared and inoculated into the Nicotiana glutinosa plant. The resulting necroses were excised, and the virus was passed through the plant two more times in a mononecrotic state, thereby biologically purifying it. After that, the virus was transferred to a host plant to propagate.