Age determination in the icefish Pseudochaenichthys georgianus (Channichthyidae) based on multiple methods using otoliths

Abstract

Aging Antarctic icefish is difficult because of their lack of scales and poorly calcified bones. Icefish ages must therefore be estimated from otoliths. We describe a method of reading daily micro-increments in connection with shape, size and mass analyses of the otoliths of the South Georgia icefish Pseudochaenichthys georgianus. Changes in otolith morphology and mass correlate with fish size and age group. The otolith micro-increment analysis is capable of establishing the age of an icefish by relating the daily micro-increment count to the life history of the fish. Micro-increment measurements and analyses are relatively simple to do by light and scanning electron microscopy and by using micro-densitometer and digitizing equipment. Drastic changes in the life history of an individual are reflected by measurable changes in its otolith micro-increment data as seen in our analyses of age groups 0-VI. The initial drastic change in daily micro-increment shapes and periodicities occur in connection with the hatching period of the icefish. The next drastic change in otolith shape and daily micro-increments occurs when ~7 cm long fish shift from pelagic to benthic habitats. As the fish age beyond group III, individual otolith variability lessens until they begin spawning. Our results indicate a single population of P. georgianus between the Antarctic Peninsula and South Georgia.