In search of new anticancer drugs: Data for cytotoxic activities of green synthesized silver nanoparticles from ethanolic extracts of fruits and leaves of Annona muricata and 5-Fluorouracil against HeLa, PC3 and PNT1A cell lines
Yahaya Gavamukulya,
Esther N. Maina,
Amos M. Meroka,
Hany A. El-Shemy,
Gabriel Magoma,
Fred Wamunyokoli
Affiliations
Yahaya Gavamukulya
Department of Molecular Biology and Biotechnology, Pan African University Institute for Basic Sciences, Technology and Innovation (PAUSTI), P. O. Box, 62000-00200 Nairobi, Kenya; Department of Biochemistry and Molecular Biology, Faculty of Health Sciences, Busitema University, P.O. Box, 1460 Mbale, Uganda; Corresponding author. Department of Molecular Biology and Biotechnology, Pan African University Institute for Basic Sciences, Technology and Innovation (PAUSTI), P. O. Box, 62000-00200, Nairobi, Kenya.
Esther N. Maina
Department of Molecular Biology and Biotechnology, Pan African University Institute for Basic Sciences, Technology and Innovation (PAUSTI), P. O. Box, 62000-00200 Nairobi, Kenya; Department of Biochemistry, College of Health Sciences, University of Nairobi, P.O. Box 30197-00100 Nairobi, Kenya
Amos M. Meroka
Department of Biochemistry, College of Health Sciences, University of Nairobi, P.O. Box 30197-00100 Nairobi, Kenya; Department of Biochemistry, School of Medicine and Health Sciences, Kenya Methodist University, P.O. Box 267-60200 Meru, Kenya
Hany A. El-Shemy
Department of Molecular Biology and Biotechnology, Pan African University Institute for Basic Sciences, Technology and Innovation (PAUSTI), P. O. Box, 62000-00200 Nairobi, Kenya; Department of Biochemistry, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt
Gabriel Magoma
Department of Molecular Biology and Biotechnology, Pan African University Institute for Basic Sciences, Technology and Innovation (PAUSTI), P. O. Box, 62000-00200 Nairobi, Kenya
Fred Wamunyokoli
Department of Molecular Biology and Biotechnology, Pan African University Institute for Basic Sciences, Technology and Innovation (PAUSTI), P. O. Box, 62000-00200 Nairobi, Kenya; Department of Biochemistry, College of Health Sciences, Jomo Kenyatta University of Agriculture and Technology, P. O. Box, 62000-00200 Nairobi, Kenya
In this article, we present data on the anticancer activities of green synthesized silver nanoparticles (AgNPs) from ethanolic extracts of fruits (AgNPs-F) and leaves (AgNPs-L) of Annona muricata and standard anticancer drug 5-Fluorouracil (5-FU) on two cancer cell lines, i.e. cervical adenocarcinoma (HeLa cells) and prostate adenocarcinoma (PC3 cells) as well as on an immortalized normal prostate cell line, PNT1A. The cytotoxicity on the cells was determined by measuring the absorbance signal of resazurin dye. It has long been known that metabolically active cells change the resazurin from blue (oxidized) to red (reduced) forms, corresponding to the absorbance signals at a wavelength of 570nm (A570) and 600nm (A600) respectively, from which therefore the effects of any treatments on percentage cell viability/death can be elucidated. The raw data values of the treatments against the HeLa, PC3 and PNT1A cells are shown in the different Tables. Examples of how the data can be analyzed have been illustrated using different growth inhibition curves. The data can be used by academics, students, and researchers working on development of anticancer drugs. Keywords: Silver nanoparticles (AgNPs), 5FU, HeLa, PC3, PNT1A, Cytotoxicity, Resazurin
