OncoTargets and Therapy (Dec 2019)

miR-30a-3p Targets MAD2L1 and Regulates Proliferation of Gastric Cancer Cells

  • Wang Y,
  • Wang F,
  • He J,
  • Du J,
  • Zhang H,
  • Shi H,
  • Chen Y,
  • Wei Y,
  • Xue W,
  • Yan J,
  • Feng Y,
  • Gao Y,
  • Li D,
  • Han J,
  • Zhang J

Journal volume & issue
Vol. Volume 12
pp. 11313 – 11324

Abstract

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Yu Wang,1,2,* Fenghui Wang,1,2,* Jing He,1,2,* Juan Du,1,2 Huahua Zhang,1,2 Haiyan Shi,1,2 Yani Chen,1,2 Yameng Wei,1,2 Wanjuan Xue,1,2 Jing Yan,1,2 Yun Feng,1,2 Yi Gao,1,2 Dan Li,1,2 Jiming Han,1,2 Jing Zhang1,2 1Department of Clinical Medicine, Medical College of Yan’an University, Yan’an, Shaanxi 716000, People’s Republic of China; 2Yan’an Key Laboratory of Chronic Disease Prevention and Research, Yan’an, Shaanxi 716000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jing ZhangDepartment of Clinical Medicine, Medical College of Yan’an University, No. 38, Guanghua Road, Yan’an 716000, People’s Republic of ChinaTel +86-911-2650158Email [email protected]: This study was done to investigate the inhibition effects of miR-30a-3p on mitotic arrest deficient 2 like 1 (MAD2L1) expression and the proliferation of gastric cancer cells.Patients and methods: Cluster analysis and the TCGA database were used to screen the key genes highly expressed in gastric cancer. Based on the LinkedOmics website, the correlation between the miR-30a-3p and the cell cycle-related target gene MAD2L1 in gastric cancer was analyzed. The mRNA and protein expression levels were detected with the quantitative real-time PCR and Western blot analysis. The cell proliferation and cell cycle were also detected and analyzed.Results: Bioinformatics analysis showed that MAD2L1 was highly expressed in tumor tissues compared with normal tissues. Compared with normal tissues, the miR-30a-3p was significantly decreased in the gastric cancer tissues. Moreover, MAD2L1 was significantly negatively correlated with the miR-30a-3p expression. Furthermore, over-expression of miR-30a-3p decreased the expression of MAD2L1 at the protein level, which inhibited the proliferation of AGS and BGC-823 gastric cancer cells. In addition, the cell cycles of AGS and BGC-823 cells were arrested at the G0/G1 phase.Conclusion: MAD2L1 is a pro-oncogene which is up-regulated in gastric cancer. The miR-30a-3p can down-regulate the MAD2L1 expression, inhibiting the proliferation of gastric cancer cells and affect the cell cycle.Keywords: gastric cancer, bioinformatics analysis, MAD2L1, miR-30a-3p, cell proliferation

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