Scientific Reports (Apr 2021)

Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics

  • Jun-Kyu Kim,
  • Young-Jin Youn,
  • Yu-Bin Lee,
  • Sun-Hwa Kim,
  • Dong-Keun Song,
  • Minsang Shin,
  • Hee Kyung Jin,
  • Jae-sung Bae,
  • Sanjeeb Shrestha,
  • Chang-Won Hong

DOI
https://doi.org/10.1038/s41598-021-87891-8
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 11

Abstract

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Abstract Extracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we investigated the possibility of neutrophils as a source for drug-loaded EVs. Neutrophil-like differentiated human promyelocytic leukemia cells (dHL-60) produced massive amounts of EVs within 1 h. The dHL-60 cells are also easily loaded with various cargoes such as antibiotics (penicillin), anticancer drug (paclitaxel), chemoattractant (MCP-1), miRNA, and Cas9. The EVs derived from the dHL-60 cells showed efficient incorporation of these cargoes and significant effector functions, such as bactericidal activity, monocyte chemotaxis, and macrophage polarization. Our results suggest that neutrophils or neutrophil-like promyelocytic cells could be an attractive source for drug-delivery EVs.