CORRECTION OF BIOLOGICAL PROPERTIES OF BIFIDOBACTERIA ISOLATED FROM HIV-INFECTED CHILDREN

Abstract

Aim. To investigate the influence of ethylmethylhydroxypyridine succinate and lysozyme on in vitro biological properties of bifidoflora isolated from HIV-positive patients. Materials and Methods. We investigated hydrophobicity, autoaggregation, and specific adhesion of 15 bifidobacteria strains from HIV-infected children after exposure to ethylmethylhydroxypyr-idine succinate, lysozyme, or both combined. Hydrophobicity was studied according to the Rosen-berg method (1980) modified by Wang (2010); autoaggregation was documented as by Del Re (2000); specific adhesion was measured in accordance with Brilis (1986). The content of fatty acids in cell walls was determined using gas chromatog-raphy-mass spectrometry. Results. Addition of ethylmethylhydroxypyridine succinate to the cultures with low antioxidant activ-ity led to the 10-fold increase in the number of Bi-dobacteria, 4-fold increase in autoaggregation, and 2-fold increase in hydrophobicity by stimulating the synthesis of unsaturated fatty acids, as compared to the control culture. Combination of ethylmethylhy-droxypyridine succinate with lysozyme elevated specific adhesion of Bifidobacteria up to 9.1%.Conclusions. The use of ethylmethylhydroxy-pyridine succinate allows to control surface properties of Bifidobacteria affecting their growth and non-specific adhesion. This may indicate usefulness of ethylmethylhydroxypyridine succinate and lysozyme for the correction of gut microbiota in HIV-infected patients.

Keywords

• вич-инфекция
• бифидобактерии
• антиоксиданты
• лизоцим
• гидрофобность
• аутоаггрегация
• hiv-infection
• bifidobacteria
• antioxidants
• lysozyme
• hydrophobicity
• autoaggregation