Nature Communications (Aug 2022)
A 33-residue peptide tag increases solubility and stability of Escherichia coli produced single-chain antibody fragments
- Yang Wang,
- Wenjie Yuan,
- Siqi Guo,
- Qiqi Li,
- Xiaomei Chen,
- Cheng Li,
- Qianying Liu,
- Lei Sun,
- Zhenguo Chen,
- Zhenghong Yuan,
- Cheng Luo,
- Shijie Chen,
- Shuping Tong,
- Michael Nassal,
- Yu-Mei Wen,
- Yong-Xiang Wang
Affiliations
- Yang Wang
- Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University
- Wenjie Yuan
- Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University
- Siqi Guo
- Drug Discovery and Design Center, the Center for Chemical Biology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
- Qiqi Li
- Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University
- Xiaomei Chen
- Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University
- Cheng Li
- Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University
- Qianying Liu
- Institutes of Biomedical Science, Fudan University
- Lei Sun
- Institutes of Biomedical Science, Fudan University
- Zhenguo Chen
- Institutes of Biomedical Science, Fudan University
- Zhenghong Yuan
- Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University
- Cheng Luo
- Drug Discovery and Design Center, the Center for Chemical Biology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
- Shijie Chen
- Drug Discovery and Design Center, the Center for Chemical Biology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
- Shuping Tong
- Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University
- Michael Nassal
- Department of Internal Medicine II/Molecular Biology, University Hospital Freiburg
- Yu-Mei Wen
- Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University
- Yong-Xiang Wang
- Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University
- DOI
- https://doi.org/10.1038/s41467-022-32423-9
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 15
Abstract
Low solubility and stability of Escherichia coli produced single chain variable fragments (scFvs) restrict their applications. Here the authors report a 33-residue peptide tag which simultaneously increases the solubility and thermostability of multiple scFvs produced in Escherichia coli SHuffle strain.
