Rosmanol controls explants browing of Hypericum canariensis L. during the in vitro estabilishment of shoots

Abstract

An efficient method for eradication of browning exudate was developed for Hypericum canariensis. For this purpose the effect of natural products on browning exudates were investigated in four types of culture media: Murashige and Skoog (MS, 1962); Gamborg's (B5, 1979); Woody Plant Medium (WPM, Lloyd and McCown 1981) and modified Quoirin and Lepoivre (QL.4) (Mederos 1991, Mederos et al. 1995) basal macroelements; these basal macroelements were supplemented with the microelement formula described by Murashige and Skoog (MS, 1962). During the establishment of shoots organogenesis potential was achieved in the Murashige and Skoog (MS, 1962) and modified Quoirin and Lepoivre (QL. 4) (Mederos 1991, Mederos et al. 1995) media after browning exudates was eliminated by rosmanol treatments. Rosmanol is a powerful diterpenic antioxidant isolated from Salvia canariensis L., a medicinal species endemic to the Canary Islands.

Keywords

• Hypericum canariensis
• browning exudate
• rosmanol antioxidant
• in vitro organogenesis