Discovery of putative tumor suppressors from CRISPR screens reveals rewired lipid metabolism in acute myeloid leukemia cells

W. Frank Lenoir; Micaela Morgado; Peter C. DeWeirdt; Megan McLaughlin; Audrey L. Griffith; Annabel K. Sangree; Marissa N. Feeley; Nazanin Esmaeili Anvar; Eiru Kim; Lori L. Bertolet; Medina Colic; Merve Dede; John G. Doench; Traver Hart

doi:10.1038/s41467-021-26867-8

Nature Communications (Nov 2021)

Discovery of putative tumor suppressors from CRISPR screens reveals rewired lipid metabolism in acute myeloid leukemia cells

W. Frank Lenoir,
Micaela Morgado,
Peter C. DeWeirdt,
Megan McLaughlin,
Audrey L. Griffith,
Annabel K. Sangree,
Marissa N. Feeley,
Nazanin Esmaeili Anvar,
Eiru Kim,
Lori L. Bertolet,
Medina Colic,
Merve Dede,
John G. Doench,
Traver Hart

Affiliations

W. Frank Lenoir: The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences; The University of Texas MD Anderson Cancer Center
Micaela Morgado: Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center
Peter C. DeWeirdt: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Megan McLaughlin: The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences; The University of Texas MD Anderson Cancer Center
Audrey L. Griffith: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Annabel K. Sangree: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Marissa N. Feeley: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Nazanin Esmaeili Anvar: The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences; The University of Texas MD Anderson Cancer Center
Eiru Kim: Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center
Lori L. Bertolet: Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center
Medina Colic: The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences; The University of Texas MD Anderson Cancer Center
Merve Dede: The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences; The University of Texas MD Anderson Cancer Center
John G. Doench: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Traver Hart: Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center

DOI: https://doi.org/10.1038/s41467-021-26867-8
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 15

Abstract

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CRISPR-based knockout screens in cancer cells have suggested the existence of proliferation suppressor genes (PSG). Here, the authors develop an approach to systematically identify them, and reveal a PSG module involved in fatty acid synthesis and tumour suppression in acute myeloid leukemia cell lines.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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