The effect of medium and plant growth regulators on micropropagation of Dog rose (Rosa canina L.)

Abstract

Dog rose (Rosa canina L.) is one of the most important ornamental and medicinal plants which are used as a rootstock for ornamental roses such as Rosa hybrid and Rosa floribunda. In vitro propagation of rose has a very important role in rapid multiplication of species with desirable traits and in production of healthy and disease-free plants. Micropropagation of Rosa canina L. was revised, using its nodal segments under different combinations of BAP (0, 0.5, 1, 1.5 and 2 mgl⁻1), GA3 (0 and 0.5 mgl⁻1) and NAA (0 and 0.5 mgl⁻1) on Murashige and Skoog (MS) and Van der Salm medium (VS) in proliferation stage and using different combinations of NAA and IBA (0, 0.3, 0.6 and 0.9 mgl⁻1) and ½ VS medium in rooting stage. The highest shoot proliferation was obtained on VS medium containing 2 mgl⁻1 BAP. Furthermore, the highest root induction obtained in ½ VS containing 0.6– 0.9 mgl⁻1 of NAA or IBA. The present study presents an in vitro protocol for R. canina.

Keywords

• Micropropagation
• native plants
• Plant growth regulators (PGRs)
• Rosa canina L