Phytochemical Screening of Different Parts of Prangos Platychlaena Boiss by Liquid-Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Abstract

Liquid-chromatography tandem mass spectrometry (LC-MS/MS) is a hyphenated analytical technique that combines the separating power of liquid chromatography with mass analysis capabilities. The aim of present study to identify and characterize different chemical compounds present in different (roots, leaves, stems and flowers fractions) parts of Prangos platychlaena Boiss. (family: Apiaceae) by LC-MS/MS method. The LC-MS/MS analysis for identification and characterization of phytocompounds in different extracts were performed, and that leads identification of five new compounds first time in P. platychlaena plant and they were psoralen, bergapten, 5,8-dimethoxy psoralen, isoimperatorin and pthalic acid mono-2-ethylhexyl ester. The identification and characterization of phytocompounds were performed on the basis on mass spectroscopy data and literature reports. The LC-MS/MS method was found simple and fast for identification and characterization of phytocompounds in different extracts of P. platychlaena.

Keywords

• prangos platychlaena
• apiaceae
• lc-ms/ms
• psoralen
• bergapten
• 5
• 8-dimethoxy psoralen
• isoimperatorin