Microorganisms (May 2020)

Advances in Molecular Tools and In Vivo Models for the Study of Human Fungal Pathogenesis

  • Dhara Malavia,
  • Neil A. R. Gow,
  • Jane Usher

DOI
https://doi.org/10.3390/microorganisms8060803
Journal volume & issue
Vol. 8, no. 6
p. 803

Abstract

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Pathogenic fungi represent an increasing infectious disease threat to humans, especially with an increasing challenge of antifungal drug resistance. Over the decades, numerous tools have been developed to expedite the study of pathogenicity, initiation of disease, drug resistance and host-pathogen interactions. In this review, we highlight advances that have been made in the use of molecular tools using CRISPR technologies, RNA interference and transposon targeted mutagenesis. We also discuss the use of animal models in modelling disease of human fungal pathogens, focusing on zebrafish, the silkworm, Galleria mellonella and the murine model.

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