Synthesis of Ultrastable Gold Nanoparticles as a New Drug Delivery System
Florence Masse,
Pascale Desjardins,
Mathieu Ouellette,
Camille Couture,
Mahmoud Mohamed Omar,
Vincent Pernet,
Sylvain Guérin,
Elodie Boisselier
Affiliations
Florence Masse
Research Center of CHU de Québec, CUO-Recherche and Université Laval, Faculty of Medicine, Ophthalmology Department, chemin Ste-Foy 1050, G1S 4L8, Québec (Québec), Canada
Pascale Desjardins
Research Center of CHU de Québec, CUO-Recherche and Université Laval, Faculty of Medicine, Ophthalmology Department, chemin Ste-Foy 1050, G1S 4L8, Québec (Québec), Canada
Mathieu Ouellette
Research Center of CHU de Québec, CUO-Recherche and Université Laval, Faculty of Medicine, Ophthalmology Department, chemin Ste-Foy 1050, G1S 4L8, Québec (Québec), Canada
Camille Couture
Research Center of CHU de Québec, CUO-Recherche and Université Laval, Faculty of Medicine, Ophthalmology Department, chemin Ste-Foy 1050, G1S 4L8, Québec (Québec), Canada
Mahmoud Mohamed Omar
Research Center of CHU de Québec, CUO-Recherche and Université Laval, Faculty of Medicine, Ophthalmology Department, chemin Ste-Foy 1050, G1S 4L8, Québec (Québec), Canada
Vincent Pernet
Research Center of CHU de Québec, CUO-Recherche and Université Laval, Faculty of Medicine, Ophthalmology Department, chemin Ste-Foy 1050, G1S 4L8, Québec (Québec), Canada
Sylvain Guérin
Research Center of CHU de Québec, CUO-Recherche and Université Laval, Faculty of Medicine, Ophthalmology Department, chemin Ste-Foy 1050, G1S 4L8, Québec (Québec), Canada
Elodie Boisselier
Research Center of CHU de Québec, CUO-Recherche and Université Laval, Faculty of Medicine, Ophthalmology Department, chemin Ste-Foy 1050, G1S 4L8, Québec (Québec), Canada
Nanotechnologies are increasingly being developed for medical purposes. However, these nanomaterials require ultrastability for better control of their pharmacokinetics. The present study describes three types of ultrastable gold nanoparticles stabilized by thiolated polyethylene glycol groups remaining intact when subjected to some of the harshest conditions described thus far in the literature, such as autoclave sterilization, heat and freeze-drying cycles, salts exposure, and ultracentrifugation. Their stability is characterized by transmission electron microscopy, UV-visible spectroscopy, and dynamic light scattering. For comparison purposes, two conventional nanoparticle types were used to assess their colloidal stability under all conditions. The ability of ultrastable gold nanoparticles to encapsulate bimatoprost, a drug for glaucoma treatment, is demonstrated. MTS assays on human corneal epithelial cells is assessed without changing cell viability. The impact of ultrastable gold nanoparticles on wound healing dynamics is assessed on tissue engineered corneas. These results highlight the potential of ultrastable gold nanoparticles as a drug delivery system in ocular therapy.
