Molecular basis for the specific and multivariant recognitions of RNA substrates by human hnRNP A2/B1

Baixing Wu; Shichen Su; Deepak P. Patil; Hehua Liu; Jianhua Gan; Samie R. Jaffrey; Jinbiao Ma

doi:10.1038/s41467-017-02770-z

Nature Communications (Jan 2018)

Molecular basis for the specific and multivariant recognitions of RNA substrates by human hnRNP A2/B1

Baixing Wu,
Shichen Su,
Deepak P. Patil,
Hehua Liu,
Jianhua Gan,
Samie R. Jaffrey,
Jinbiao Ma

Affiliations

Baixing Wu: State Key Laboratory of Genetic Engineering, Collaborative Innovation Centre of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University
Shichen Su: State Key Laboratory of Genetic Engineering, Collaborative Innovation Centre of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University
Deepak P. Patil: Department of Pharmacology, Weill Cornell Medicine, Cornell University
Hehua Liu: State Key Laboratory of Genetic Engineering, Collaborative Innovation Centre of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University
Jianhua Gan: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan University
Samie R. Jaffrey: Department of Pharmacology, Weill Cornell Medicine, Cornell University
Jinbiao Ma: State Key Laboratory of Genetic Engineering, Collaborative Innovation Centre of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University

DOI: https://doi.org/10.1038/s41467-017-02770-z
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 12

Abstract

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RNA-binding protein hnRNP A2/B1 is suggested to promote miRNA processing as a m6A 'reader'. Here, the authors determine crystal structures of RRM domains of hnRNP A2/B1 in complex with various RNA substrates and determine that hnRNP A2/B1 may function as an auxiliary factor in 'm6A switch' instead.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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