Brain Sciences (Apr 2023)

DEAD-Box Helicase 17 Promotes Amyloidogenesis by Regulating BACE1 Translation

  • Yue Liu,
  • Guifeng Zhou,
  • Li Song,
  • Qixin Wen,
  • Shiqi Xie,
  • Long Chen,
  • Lu Wang,
  • Xiaoyong Xie,
  • Xue Chen,
  • Yalan Pu,
  • Guojun Chen

DOI
https://doi.org/10.3390/brainsci13050745
Journal volume & issue
Vol. 13, no. 5
p. 745

Abstract

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Amyloidogenesis is one of the key pathophysiological changes in Alzheimer’s disease (AD). Accumulation of the toxic Aβ results from the catalytic processing of β-amyloid precursor protein (APP) associated β-amyloid converting enzyme 1 (BACE1) activity. It is reported that dead-box helicase 17 (DDX17) controls RNA metabolism and is involved in the development of multiple diseases. However, whether DDX17 might play a role in amyloidogenesis has not been documented. In the present study, we found that DDX17 protein level was significantly increased in HEK and SH-SY5Y cells that stably express full-length APP (HEK-APP and Y5Y-APP) and in the brain of APP/PS1 mice, an animal model of AD. DDX17 knockdown, as opposed to DDX17 overexpression, markedly reduced the protein levels of BACE1 and the β-amyloid peptide (Aβ) in Y5Y-APP cells. We further found that DDX17-mediated enhancement of BACE1 was selectively attenuated by translation inhibitors. Specifically, DDX17 selectively interacted with the 5′ untranslated region (5′UTR) of BACE1 mRNA, and deletion of the 5′UTR abolished the effect of DDX17 on luciferase activity or protein level of BACE1. Here, we show that the enhanced expression of DDX17 in AD was associated with amyloidogenesis; through the 5′UTR-dependent BACE1 translation, DDX17 might serve as an important mediator contributing to the progression of AD.

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