Purified F-ATP synthase forms a Ca2+-dependent high-conductance channel matching the mitochondrial permeability transition pore

Andrea Urbani; Valentina Giorgio; Andrea Carrer; Cinzia Franchin; Giorgio Arrigoni; Chimari Jiko; Kazuhiro Abe; Shintaro Maeda; Kyoko Shinzawa-Itoh; Janna F. M. Bogers; Duncan G. G. McMillan; Christoph Gerle; Ildikò Szabò; Paolo Bernardi

doi:10.1038/s41467-019-12331-1

Nature Communications (Sep 2019)

Purified F-ATP synthase forms a Ca2+-dependent high-conductance channel matching the mitochondrial permeability transition pore

Andrea Urbani,
Valentina Giorgio,
Andrea Carrer,
Cinzia Franchin,
Giorgio Arrigoni,
Chimari Jiko,
Kazuhiro Abe,
Shintaro Maeda,
Kyoko Shinzawa-Itoh,
Janna F. M. Bogers,
Duncan G. G. McMillan,
Christoph Gerle,
Ildikò Szabò,
Paolo Bernardi

Affiliations

Andrea Urbani: Department of Biomedical Sciences, University of Padova
Valentina Giorgio: Department of Biomedical Sciences, University of Padova
Andrea Carrer: Department of Biomedical Sciences, University of Padova
Cinzia Franchin: Department of Biomedical Sciences, University of Padova
Giorgio Arrigoni: Department of Biomedical Sciences, University of Padova
Chimari Jiko: Institute for Integrated Radiation and Nuclear Science, Kyoto University
Kazuhiro Abe: Cellular and Structural Physiology Institute, Nagoya University
Shintaro Maeda: Department of Integrative Structural and Computational Biology, Scripps Research Institute
Kyoko Shinzawa-Itoh: Department of Life Science, Graduate School of Life Science, University of Hyogo
Janna F. M. Bogers: Department of Biotechnology, Delft University of Technology
Duncan G. G. McMillan: Department of Biotechnology, Delft University of Technology
Christoph Gerle: Institute for Protein Research, Osaka University
Ildikò Szabò: Consiglio Nazionale delle Ricerche Neuroscience Institute
Paolo Bernardi: Department of Biomedical Sciences, University of Padova

DOI: https://doi.org/10.1038/s41467-019-12331-1
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 11

Abstract

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The molecular identity of the mitochondrial megachannel (MMC)/permeability transition pore (PTP), a key effector of cell death, remains controversial. Here authors demonstrate that the membrane embedded bovine F-ATP synthase elicits Ca2 + -dependent currents matching those of the MMC/PTP.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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