Transforming protein-polymer conjugate purification by tuning protein solubility

Stefanie L. Baker; Aravinda Munasinghe; Bibifatima Kaupbayeva; Nin Rebecca Kang; Marie Certiat; Hironobu Murata; Krzysztof Matyjaszewski; Ping Lin; Coray M. Colina; Alan J. Russell

doi:10.1038/s41467-019-12612-9

Nature Communications (Oct 2019)

Transforming protein-polymer conjugate purification by tuning protein solubility

Stefanie L. Baker,
Aravinda Munasinghe,
Bibifatima Kaupbayeva,
Nin Rebecca Kang,
Marie Certiat,
Hironobu Murata,
Krzysztof Matyjaszewski,
Ping Lin,
Coray M. Colina,
Alan J. Russell

Affiliations

Stefanie L. Baker: Department of Biomedical Engineering, Scott Hall 4N201, Carnegie Mellon University
Aravinda Munasinghe: Department of Chemistry, 354 Leigh Hall, University of Florida
Bibifatima Kaupbayeva: Center for Polymer-Based Protein Engineering, Carnegie Mellon University
Nin Rebecca Kang: Department of Biomedical Engineering, Scott Hall 4N201, Carnegie Mellon University
Marie Certiat: Department of Chemistry, 354 Leigh Hall, University of Florida
Hironobu Murata: Center for Polymer-Based Protein Engineering, Carnegie Mellon University
Krzysztof Matyjaszewski: Center for Polymer-Based Protein Engineering, Carnegie Mellon University
Ping Lin: Department of Chemistry, 354 Leigh Hall, University of Florida
Coray M. Colina: Department of Chemistry, 354 Leigh Hall, University of Florida
Alan J. Russell: Department of Biomedical Engineering, Scott Hall 4N201, Carnegie Mellon University

DOI: https://doi.org/10.1038/s41467-019-12612-9
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 12

Abstract

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Therapeutic proteins are often conjugated with polymers, but separating the conjugate from unconjugated protein and free polymer is a major challenge. Here, the authors discover that proteins conjugated to charged or zwitterionic polymers maintain solubility in 100% ammonium sulfate, greatly simplifying purification.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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